ANC28.1 mAb as a stimulating agent in a Cytokine Release Assay for assessment of preclinical safety

“Development of a flow cytometry-based potency assay for prediction of cytokine storms induced by biosimilar monoclonal antibodies” Natasha Helleberg Madsen, Monika Gad, Jesper Larsen. (Feb 5, 2022) J Immunol Methods 502: 113231. https://doi.org/10.10 PBMC were stimulated with immobilized ANC28.1, CD28.1 and muromonab CD3. Assessed Cytokines and levels of Activation molecules by flow could determine the …

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Ancell reagents and personnel involved in a new SARS-Co-V2 breast milk antibody study

“Increase in SARS-CoV-2 RBD-specific IgA and IgG Antibodies in Breast Milk from Lactating Women Following the COVID-19 Booster Vaccination” Andrea M Henle MedRxiv (Feb 24, 2022 Pre print) doi: https://doi.org/10.1101/2022.02.23.22271414 Related Ancell products: anti-human IgA monoclonal antibody anti-human IgG monoclonal antibody Recombinant SARS-CO-V2-RBD-His protein #581-820 (Inquire)

Exosomes increase PD-L1 expression and induce tolerance to NIMA

A University of WI Madison research group used Ancell antibodies to analyze exosomes involved in the induction of tolerance to non-inherited maternal antigens(NIMA). This could have implications for allogeneic graft tolerance./ “Cross-Decoration of Dendritic Cells by Non-Inherited Maternal Antigen-Containing Extracellular Vesicles: Potential Mechanism for PD-L1-Based Tolerance in Cord Blood and Organ Transplantation”Diego A. Lema, A …

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Ancell anti-CD63 antibody used to capture Tumor Extracellular Vesicles from Colorectal Cancer patient plasma in a high throughput device

The device captures EV from plasma and analyzes clinically relevant tumour biomarkers (EGFR, EpCAM, CD24 and GPA33) of colorectal cancer (CRC). The results were predictive of disease severity and prognosis for the test subjects. “An integrated magneto-electrochemical device for the rapid profiling of tumour extracellular vesicles from blood plasma” Jongmin Park, Hakho Lee, et al. …

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Neutrophil Trogocytosis blocked by Ancell anti-CD18 mAb and F(ab’)2 reagents in a CD47-SIRPa checkpoint disrupted model

The use of Neutrophils from FERMT3 patients elucidated the requirement of kindlen3-CD18 interaction for synapse formation and trogocytosis of antibody opsinized SIRPa-CD47 checkpoint disruped cancer cells in vitro. “Kindlin3-Dependent CD11b/CD18-Integrin Activation IsRequired for Potentiation of Neutrophil Cytotoxicity byCD47–SIRPa Checkpoint Disruption” Panagiota Bouti, Timo K. van den Berg, et al. (Dec 2020) Cancer Immunology Research Priority …

Neutrophil Trogocytosis blocked by Ancell anti-CD18 mAb and F(ab’)2 reagents in a CD47-SIRPa checkpoint disrupted model Read More »

TRCB1 (T cell Receptor C Beta 1) is proving to be an increasingly useful cell surface marker in identification of T cell subsets and T cell malignancies

  “Flow cytometric evaluation of TRBC1 expression in tissue specimens and body fluids is a novel and specific method for assessment of T-cell clonality and diagnosis of T-cell neoplasms”   H Berg, H Olteanu, et al. (May 2021) Clinical Cytometry 100(3): 361-369. https://doi.org/10.1002/cyto.b.21881   Ancell anti-human TRCB1 (Clone JOVI-1) reagents: 101-020 Purified (with Preservative) 101-820 Purified …

TRCB1 (T cell Receptor C Beta 1) is proving to be an increasingly useful cell surface marker in identification of T cell subsets and T cell malignancies Read More »

TIGIT blockade restores T cell function in pancreatic cancer model

Blockade of CD155/TIGIT signaling in conjunction with PD-1 blockade and CD40 agonism restores T cell function in mouse pancreatic cancer model “The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer” Freed-Pastor WA, Tyler Jacks, et al. (July 2021) Cancer Cell S1535-6108(21)00384-6. doi: 10.1016/j.ccell.2021.07.007 Relevant Ancell Immunology tools anti-human TIGIT mAb Recombinant human …

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Recombinant CD304(Neuropilin) now availible!

Highly active recombinant CD304-muIg protein now available from Ancell! Ancell HEK cell derived CD304-muIg binds in EIA to immobilized VEGF165. CD304 references “CD304/neuropilin-1 is a very useful and dependable marker for the measurable residual disease assessment of B-cell precursor acute lymphoblastic leukemia” Pratyusha Gudapati Prashant R Tembhare , et al. (2020 Jul) Cytometry B Clin Cytom. …

Recombinant CD304(Neuropilin) now availible! Read More »