anti-CD70 FITC Conjugate

Human CD70 is not present on resting lymphocytes.  CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells.  CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family (3). 

Isotype:  Murine IgG1

Immunogen: Human WM-1 (Waldenström’s macroglobulinemia) cell line

Specificity: Antibody BU69 recognizes the CD70 molecule of approximately 30 kd (3).

Functional Application: Antibody BU69 blocks the interaction between CD27 and CD70, and has been shown to  inhibit T cell proliferation induced by dendritic cells(5).

References:

1.  R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773.

2.  M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761.

3.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138.

4. K. Agematsu, et al, (1995) J Immunol  154: 3627-3635.

5. S Iwamoto, A Miyazaki, et al, (2005) J Leuk Biol 78: 383-392.

6. Wischhusen J, M Weller, et al. (2002) Cancer Res 62: 2592-2599

7. P.J. Adams, A.L. Harris, et al.(2006) British J Cancer 95:298-306.

8. C Muller, R Kuppers, et al.(2010) Intl Immunol 22(4):289-298.

9.  H Yokoi, H Yoshikawa, et al (2003) Lymphology 36: 3674-83. PMID: 12926832