anti-CD137 F(ab')2 (Preservative-free)

Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3).  CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2).  CD137 appears to be important for T cell proliferation and survival (4) and induces monocyte activation (5).

Isotype: Murine IgG1 kappa

Immunogen: Ectodomain of recombinant human 4-1BB fusion protein

Specificity: Antibody 4B4-1 recognizes the CRDII extracellular epiopte (8) of the human CD137 molecule of about 35 kd on activated T cells (3).

Functional Application: Antibody 4B4-1 interferes with binding of Biotinylated Recombinant CD137-huIg (502-030) to CD137L on Raji cells, but does not block binding of Recombinant CD137L-muCD8 (503-020)  to CD137 on surface of stimulated CEM cells.   Immobilized mAb has been shown to induce TNF a production by monocytes(6) and IFNg production by NK cells(7).

References:

1.  M.R. Alderson, et al, (1994) Eur J Immunol  24: 2219-2227.

2.  H.J. Gruss, and S.K. Dower, (1995) Blood  85: 3378-3404.

3.  B.A. Garni-Wagner, et al, (1996) Cellular Immunol  169: 91-98.

4.  H. Schwarz, et al, (1996) Blood  87: 2839-2845.

5.  J. Langstein, H. Schwartz, et al, (1998) J Immunol  160: 2488-2494.

6.  G. Kienzle, J. von Kempis, (1999) Int Immunol 12(1): 73-82.

7.  Baessler T, H R Salih, et al. (2009) 115(15): 3058-3069.

8.  Ling Yi, Hougtao Zhang, et al. (Jan 2014) PLOS One. https://doi.org/10.1371/journal.pone.0086337

USD $720.00
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Catalog #:
360-520
Form:
F(ab')2
Size:
100 µg
Alternate Name:
4-1BB
Clone:
4B4-1
Applications:
EIA, FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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