Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3). CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2). CD137 appears to be important for T cell proliferation and survival (4) and induces monocyte activation (5).
Isotype: Murine IgG1 kappa
Immunogen: Ectodomain of recombinant human 4-1BB fusion protein
Specificity: Antibody 4B4-1 recognizes the CRDII extracellular epiopte (8) of the human CD137 molecule of about 35 kd on activated T cells (3).
Functional Application: Antibody 4B4-1 interferes with binding of Biotinylated Recombinant CD137-huIg (502-030) to CD137L on Raji cells, but does not block binding of Recombinant CD137L-muCD8 (503-020) to CD137 on surface of stimulated CEM cells. Immobilized mAb has been shown to induce TNF a production by monocytes(6) and IFNg production by NK cells(7).
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2. H.J. Gruss, and S.K. Dower, (1995) Blood 85: 3378-3404.
3. B.A. Garni-Wagner, et al, (1996) Cellular Immunol 169: 91-98.
4. H. Schwarz, et al, (1996) Blood 87: 2839-2845.
5. J. Langstein, H. Schwartz, et al, (1998) J Immunol 160: 2488-2494.
6. G. Kienzle, J. von Kempis, (1999) Int Immunol 12(1): 73-82.
7. Baessler T, H R Salih, et al. (2009) 115(15): 3058-3069.
8. Ling Yi, Hougtao Zhang, et al. (Jan 2014) PLOS One. https://doi.org/10.1371/journal.pone.0086337