anti-CD137 Biotinylated

Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3).  CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2).  CD137 appears to be important for T cell proliferation and survival (4) and induces monocyte activation (5).

Isotype: Murine IgG1 kappa

Immunogen: Ectodomain of recombinant human 4-1BB fusion protein

Specificity: Antibody 4B4-1 recognizes the CRDII extracellular epiopte (8) of the human CD137 molecule of about 35 kd on activated T cells (3).

Functional Application: Antibody 4B4-1 interferes with binding of Biotinylated Recombinant CD137-huIg (502-030) to CD137L on Raji cells, but does not block binding of Recombinant CD137L-muCD8 (503-020)  to CD137 on surface of stimulated CEM cells.   Immobilized mAb has been shown to induce TNF a production by monocytes(6) and IFNg production by NK cells(7).

References:

1.  M.R. Alderson, et al, (1994) Eur J Immunol  24: 2219-2227.

2.  H.J. Gruss, and S.K. Dower, (1995) Blood  85: 3378-3404.

3.  B.A. Garni-Wagner, et al, (1996) Cellular Immunol  169: 91-98.

4.  H. Schwarz, et al, (1996) Blood  87: 2839-2845.

5.  J. Langstein, H. Schwartz, et al, (1998) J Immunol  160: 2488-2494.

6.  G. Kienzle, J. von Kempis, (1999) Int Immunol 12(1): 73-82.

7.  Baessler T, H R Salih, et al. (2009) 115(15): 3058-3069.

8.  Ling Yi, Hougtao Zhang, et al. (Jan 2014) PLOS One. https://doi.org/10.1371/journal.pone.0086337