anti-CD152 (F(ab’)2)

Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells (1). CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation (2, 3).

Isotype: Murine IgG1kappa

Immunogen: Stimulated human PBL’s and human CD152 Ig fusion protein

Specificity: Antibody ANC152.2/8H5 reacts with an extracellular epitope of human CTLA-4 expressed on activated T cells. It has been reported to cross react with dog (11, 12), pig, cow and cat CD152.

Functional Application: ANC152.2 blocks binding of CD152 (CTLA-4) Ig fusion protein to its CD80/CD86 receptor, and has been reported to modulate suppressive function of human T Regulatory Cells in Vitro (4). ANC152.2 can bind to intracellular CD152 in fixed permeabolized cells. Purified and F(ab’)2 forms of this clone have been used to block proliferation and/or IL-10 production by activated T cells in various models (5, 6, 7, 8, 9) . ANC152.2 blockade upregulated Activation Induced Marker profile in an Acute Coronary Syndrome model (13).

References:

1) T.L. Walunas, et al, (1994) Immunity 1:405-413.

2) N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788.

3) A. Kitani, et al, (2000) J Immunol 165: 691-702.

4) Y. Zheng, D.M. Sansom, et al, (2004)

5) S Vendetti, M.T. DeMagistris, et al. (2008) Immunology Letters 115(1):59-69.

6) F J Ward, R N Barker, et al. (2002) Blood 1004529-4536.

7) F J Ward, R N Barker, et al. (2008) Blood 111: 680-687.

8) Wenwei Tu, D BLewis, et al. (2008) Blood 112: 2554-2562.

9. I Bellinghausen, J Saloga, et al (2003) J Allergy Clin Immunol 111(4): 862-868. PMID: 12704370

10. Yang, N., Li, J., Yang, Q. et al. Arch Virol (2018). https://doi.org/10.1007/s00705-018-3735-8

11. Tagawa M, S Takagi, et al. (Feb 2016) PLoS One 11(2): e0150030. https://doi.org/10.1371/journal.pone.0150030