anti-CD152 R-PE Conjugate

Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells (1).  CD152  is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation (2, 3).  

Isotype:  Murine IgG1kappa 

Immunogen:  Stimulated human PBL’s and human CD152 Ig fusion protein

Specificity:  Antibody ANC152.2/8H5 reacts with an extracellular epitope of human CTLA-4 expressed on activated T cells.  It has been reported to cross react with dog (11, 12), pig, cow and cat CD152.

 Functional Application:  ANC152.2 blocks binding of CD152 (CTLA-4) Ig fusion protein to its CD80/CD86 receptor, and has been reported to modulate suppressive function of human T Regulatory Cells in Vitro (4). ANC152.2 can bind to intracellular CD152 in fixed permeabolized cells.  Purified and  F(ab’)2 forms of this clone have been used to block  proliferation and/or IL-10 production by activated T cells in various models (5, 6, 7, 8, 9) .

References: 

1) T.L. Walunas, et al, (1994) Immunity  1:405-413. 

2) N.J. Karandikar, et al, (1996) J Exp Med  184: 783-788. 

3) A. Kitani, et al, (2000) J Immunol  165: 691-702.

4) Y. Zheng, D.M. Sansom, et al, (2004)

5) S Vendetti, M.T. DeMagistris, et al. (2008) Immunology Letters 115(1):59-69.

6) F J Ward, R N Barker, et al. (2002) Blood 1004529-4536.

7) F J Ward, R N Barker, et al. (2008) Blood 111: 680-687.

8) Wenwei Tu, D BLewis, et al. (2008) Blood 112: 2554-2562.

9. I Bellinghausen, J Saloga, et al (2003) J Allergy Clin Immunol 111(4): 862-868. PMID: 12704370

10. Yang, N., Li, J., Yang, Q. et al. Arch Virol (2018). https://doi.org/10.1007/s00705-018-3735-8

11. Tagawa M, S Takagi, et al. (Feb 2016) PLoS One 11(2): e0150030.   https://doi.org/10.1371/journal.pone.0150030

12. Tagawa M, K Miyahara, et al. (July 2018) PLoS ONE 13(7): e0201222.https://doi.org/10.1371/journal. pone.0201222.

USD $325.00
In cart: 0
= $325.00
Catalog #:
359-050
Form:
R-PE
Size:
120 tests
Alternate Name:
CTLA-4
Clone:
ANC152.2/8H5
Applications:
Blocking, EIA, FC(10), FX

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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