anti-CD106 FITC Conjugate
Human CD106 is an endothelial adhesion molecule that binds to alpha 4 beta 1 & alpha 4 beta 7 integrins (VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils. CD106 is also found on follicular dendritic cells, interdigitating recticulum cells and Kupffer cells (3).
Isotype: Murine IgG1
Immunogen: Human endothelial cells
Specificity: Antibody 1.G11B1 recognizes the an extracellular epitope of the human VCAM-1 molecule (CD106) which is involved with leukocyte adhesion (1, 2,3). It has been reported to be cross reactive with porcine CD106.
Functional Application: Antibody 1.G11B1 binds to an epitope located within the first three n-terminal loops of CD106. This mAb blocks lymphocyte(3,12) and eosinophil (6) adhesion to CD106. It has been reported to work in FACS(10, 11), IHC (frozen section) and Western blot(8).
References:
1) S M Wellicome SM, Haskard DO et al. (1990) J Immunol 144(7):2558-65.
2) M.H. Thornhill, D.O. Haskard, (1991) J Immunol 146: 592-598.
3) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1764-1767.
4) M. Nagata, et al, (1995) J Immunol 155: 2194-2202.
5) H.E. Chuluyan, et al, (1995) J Immunol 155: 3135-3144.
6) K.D. Patel (1998) Blood 92 (10): 3904-3911.
7) S.R. Barthel, M.W. Johansson, et al. (2006) J Biol Chem 281(43): 32175-32187.
8) H Valentin, L Gazzolo, et al. (1997) J Virol 71(11): 8522-8530.
9) A Maenpaa, T Timonen, et al. (1997) ACTA Neuropathologica 94(3):216-225.
10) H Yarwood, D O Haskard, et al. (2000) J Leukoc Biol 68: 233–242.
12) XY Zhou, ZC Han, et al.(2017) Exp Ther Med 14(4): 3541-48. doi.org/10.3892/etm.2017.4952