anti-CD106 Biotinylated

Human CD106 is an endothelial adhesion molecule that binds to alpha 4 beta 1 & alpha 4 beta 7 integrins (VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils.  CD106 is also found on follicular dendritic cells, interdigitating recticulum cells and Kupffer cells (3).

Isotype: Murine IgG1

Immunogen: Human endothelial cells

Specificity: Antibody 1.G11B1 recognizes the an extracellular epitope of the human VCAM-1 molecule (CD106) which is involved with leukocyte adhesion (1, 2,3). It has been reported to be cross reactive with porcine CD106.

Functional Application: Antibody 1.G11B1 binds to an epitope located within the first three  n-terminal loops of CD106.  This mAb blocks lymphocyte(3,12) and eosinophil (6) adhesion to CD106. It has been reported to work in FACS(10, 11), IHC (frozen section) and Western blot(8).

References:

1)   S M Wellicome SM, Haskard DO et al. (1990)  J Immunol 144(7):2558-65.

2)  M.H. Thornhill, D.O. Haskard, (1991) J Immunol  146: 592-598.

3)  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1764-1767.

4)  M. Nagata, et al, (1995) J Immunol  155: 2194-2202.

5)  H.E. Chuluyan, et al, (1995) J Immunol  155: 3135-3144.

6)  K.D. Patel (1998) Blood 92 (10): 3904-3911.

7)  S.R. Barthel, M.W. Johansson, et al. (2006) J Biol Chem 281(43): 32175-32187.

8)  H Valentin, L Gazzolo, et al. (1997) J Virol 71(11): 8522-8530.

9)  A Maenpaa, T Timonen, et al. (1997) ACTA Neuropathologica 94(3):216-225.

10)  H Yarwood,  D O Haskard, et al. (2000) J Leukoc Biol 68: 233–242.

11)  AF Tramontano, L Ragolia, et al. (2010) Mediators of Inflammation 2010: 250476  doi:10.1155/2010/250476.

12) XY Zhou, ZC Han, et al.(2017) Exp Ther Med 14(4): 3541-48. doi.org/10.3892/etm.2017.4952

USD $210.00
In cart: 0
= $210.00
Catalog #:
327-030
Form:
Biotin
Size:
100 µg
Alternate Name:
VCAM-1
Clone:
1.G11B1
Applications:
6), Blocking(3, EIA, FC, IHC, WB(8)

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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