Human IgG1 Biotinylated

Human IgG1k from human myeloma plasma is used as a negative control(1,2) for work involving human IgG1 isotype antibodies or recombinant proteins.

The molecular weight of this myeloma derived protein has varied for some lots; running at about 135 kD in SDS-PAGE, compared to the expected 150 kD for normal human IgG1.  This was due to a smaller heavy chain, which ran at approximately 40 kD when reduced.

 

References

1) J Shen, PS Low, et al. (2014) J Leuk Biol 96: 563-570. PMID: 25015955

2) C E Green, S I Simon, et al.(2003) Am J Physiol – Cell Physiol 284(3): C705-C717.

3) S Watanabe, S G Vasudevan, et al.(2015) J Virol 89(11): 5847-61.

USD $275.00
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= $275.00
Catalog #:
295-030
Form:
Biotin
Size:
100 µg
Alternate Name:
huIgG1k, human IgG1 kappa
Clone:
Myeloma
Applications:
EIA, FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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