Human IgG1 Allophycocyanin

Human IgG1k from human myeloma plasma is used as a negative control(1,2) for work involving human IgG1 isotype antibodies or recombinant proteins.

The molecular weight of this myeloma derived protein has varied for some lots; running at about 135 kD in SDS-PAGE, compared to the expected 150 kD for normal human IgG1.  This was due to a smaller heavy chain, which ran at approximately 40 kD when reduced.

Ancell huIgG1k/Biotin has been used in a complexed form to stimulate IL-6 and IgG production by human PBMC(4).

 

References

1) J Shen, PS Low, et al. (2014) J Leuk Biol 96: 563-570. PMID: 25015955

2) C E Green, S I Simon, et al.(2003) Am J Physiol – Cell Physiol 284(3): C705-C717.

3) S Watanabe, S G Vasudevan, et al.(2015) J Virol 89(11): 5847-61.

4) Aripita Deb, BL Kaplan, et al. (4 May, 2026) Antibodies 15(4): 68. PMID: 42645425 DOI: 10.3390/antib15040068