anti-CD39 Biotinylated

Human CD39 is found on most mature B cells, activated NK cells and activated T cells, as well as Neutrophills and Monocytes(7).  CD39 has homology to the N- myc family of proteins (1) and has been cloned (2). CD39 is an Ecto Apyrase(4, 6). A lower expression level of CD39 on T  and NK cells have been observed in hypertensive individuals(7). 

Isotype: Murine IgG1

Immunogen: Human WM-1 (Waldenström’s macroglobulinemia) cell line

Specificity: Antibody BU61 recognizes the CD39 molecule of approximately 80 kd (3).  A deletion mutation study suggests that the epitope of BU61 is between amino acids 329 and 427 (11).

Applications include Immunohistochemistry (frozen section) (8), Western Blot (4,5,8,10) , Flow Cytometry, and Immunoprecipitation (3).

References:

1.  J.R. Voland, et al, (1992) Proc Natl Acad Sci USA 89: 10425-10429.

2.  C.R. Maliszewski, et al, (1994) J Immunol  153: 3574-3583.

3.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 383-385.

4.  E. Kaczmarek, et al, (1996) J Biol Chem  271:33116-33122.

5. K. Koziak, S.C. Robson, et al, (2000)J Biol Chem 275(3): 2057-2062.

6. Transfusion (May 2006) 46(6):1018-1028.

7. E D Pulte, A J Marcus, et al. (2007) Thrombosis Res 121(3): 309-317.

8. S Sheth, S. C. Robson, et al. (2009) Purinergenic Signalling   5(3): 321-326.

9. “Cholesterol-dependent Lipid Assemblies Regulate the Activity of the Ecto-nucleotidase CD39.”  A. Papanikolaou,  S. Christoforidis, et al.(July 15, 2005)  Journal of Biological Chemistry 280: 26406-26414.

10.  C A Haller, E L Chaikof, et al. (2006) J Vascular Surgery 43(4): 816-23. PMID: 16616242

11.  J Schulte am Esch II, S C Robson, et al. (1999) Biochemistry38(8): 2248-58. PMID: 10029517.