anti-CD39 Purified (with Antibiotics)

Human CD39 is found on most mature B cells, activated NK cells and activated T cells, as well as Neutrophills and Monocytes(7).  CD39 has homology to the N- myc family of proteins (1) and has been cloned (2). CD39 is an Ecto Apyrase(4, 6). A lower expression level of CD39 on T  and NK cells have been observed in hypertensive individuals(7). 

Isotype: Murine IgG1

Immunogen: Human WM-1 (Waldenström’s macroglobulinemia) cell line

Specificity: Antibody BU61 recognizes the CD39 molecule of approximately 80 kd (3).  A deletion mutation study suggests that the epitope of BU61 is between amino acids 329 and 427 (11).

Applications include Immunohistochemistry (frozen section) (8), Western Blot (4,5,8,10) , Flow Cytometry, and Immunoprecipitation (3).

References:

1.  J.R. Voland, et al, (1992) Proc Natl Acad Sci USA 89: 10425-10429.

2.  C.R. Maliszewski, et al, (1994) J Immunol  153: 3574-3583.

3.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 383-385.

4.  E. Kaczmarek, et al, (1996) J Biol Chem  271:33116-33122.

5. K. Koziak, S.C. Robson, et al, (2000)J Biol Chem 275(3): 2057-2062.

6. Transfusion (May 2006) 46(6):1018-1028.

7. E D Pulte, A J Marcus, et al. (2007) Thrombosis Res 121(3): 309-317.

8. S Sheth, S. C. Robson, et al. (2009) Purinergenic Signalling   5(3): 321-326.

9. “Cholesterol-dependent Lipid Assemblies Regulate the Activity of the Ecto-nucleotidase CD39.”  A. Papanikolaou,  S. Christoforidis, et al.(July 15, 2005)  Journal of Biological Chemistry 280: 26406-26414.

10.  C A Haller, E L Chaikof, et al. (2006) J Vascular Surgery 43(4): 816-23. PMID: 16616242

11.  J Schulte am Esch II, S C Robson, et al. (1999) Biochemistry38(8): 2248-58. PMID: 10029517.

USD $175.00
In cart: 0
= $175.00
Catalog #:
188-020
Form:
Pur/WA
Size:
100 µg
Alternate Name:
EctoApyrase
Clone:
BU61
Applications:
5)), 8), FC, IHC(8), IP(3), WB (4

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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