Human CD39 is found on most mature B cells, activated NK cells and activated T cells, as well as Neutrophills and Monocytes(7). CD39 has homology to the N- myc family of proteins (1) and has been cloned (2). CD39 is an Ecto Apyrase(4, 6). A lower expression level of CD39 on T and NK cells have been observed in hypertensive individuals(7).
Isotype: Murine IgG1
Immunogen: Human WM-1 (Waldenström’s macroglobulinemia) cell line
Specificity: Antibody BU61 recognizes the CD39 molecule of approximately 80 kd (3). A deletion mutation study suggests that the epitope of BU61 is between amino acids 329 and 427 (11).
Applications include Immunohistochemistry (frozen section) (8), Western Blot (4,5,8,10) , Flow Cytometry, and Immunoprecipitation (3).
1. J.R. Voland, et al, (1992) Proc Natl Acad Sci USA 89: 10425-10429.
2. C.R. Maliszewski, et al, (1994) J Immunol 153: 3574-3583.
3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 383-385.
6. Transfusion (May 2006) 46(6):1018-1028.
9. “Cholesterol-dependent Lipid Assemblies Regulate the Activity of the Ecto-nucleotidase CD39.” A. Papanikolaou, S. Christoforidis, et al.(July 15, 2005) Journal of Biological Chemistry 280: 26406-26414.