CD304-muIg Purified (with Antibiotics)
INFORMATION: Human CD304 (neuropilin, NRP, NP1, BDCA-4) is a 140 kD type I glycoprotein expressed by a variety of tissue types,including Treg, follicular TH, neurons, Dendritic cells, and Endothelial cells. Its ligands include VEGF165 and Semaphorin family members. It is a reliable marker for human BCP-ALL1. Soluble CD304 has been used to slow progression of murine AML 2. CD304-muIg runs as a dimer in SDS-PAGE with a molecular weight of approximately 200 kD.
Molecular Structure: A soluble molecule consisting of the extracellular domain of mature human CD304 fused to murine IgG2a Fc.
Residual signal peptide amino acids (6aa): kpqape
Mature CD304(EC) (623aa): (22)frndkcgdtikiespgyltspgyphsyhpsekcewliqapdpyqriminfnphfdledrdckydyvevfdgenenghfrgkfcgkiapppvvssgpflfikfvsdyethgagfsiryeifkrgpecsqnyttpsgvikspgfpekypnslectyivfvpkmseiilefesfdlepdsnppggmfcrydrleiwdgfpdvgphigrycgqktpgrirsssgilsmvfytdsaiakegfsanysvlqssvsedfkcmealgmesgeihsdqitassqystnwsaersrlnypengwtpgedsyrewiqvdlgllrfvtavgtqgaisketkkkyyvktykidvssngedwitikegnkpvlfqgntnptdvvvavfpkplitrfvrikpatwetgismrfevygckitdypcsgmlgmvsglisdsqitssnqgdrnwmpenirlvtsrsgwalppaphsyinewlqidlgeekivrgiiiqggkhrenkvfmrkfkigysnngsdwkmimddskrkaksfegnnnydtpelrtfpalstrfiriyperathgglglrmellgceveaptagpttpngnlvdecdddqanchsgtgddfqltggttvlatekptvidstiqsefp(644)
Murine IgG2aFc (233aa): eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk
Predicted nonglycosylated monomeric weight: 97 kd.
Functional Application: Recombinant CD304-muIg binds to immobilized VEGF165 in EIA.
Transfectant Cell Line: HEK
References:
1) Pratyusha Gudapati Prashant R Tembhare , et al. (2020 Jul) Cytometry B Clin Cytom. 98(4):328-335. doi: 10.1002/cyto.b.21866. Epub 2020 Jan 16.