CD252(OX40L)-muCD8 Purified (with Antibiotics)

Human CD252 (CD134L, OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells.  CD252 binds to CD134 present on  activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of  immflamation and rejection (5,6,7,8).  CD252-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.

Molecular Structure:  A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 132 (c-term) amino acids of the extracellular domain of human CD252, with a predicted (non glycosylated) monomeric weight of 34.1 kd.   Extracellular mature murine CD8alpha: (28)KPQAPEL….GLDFACD(194); Linking amino acids: TR; extracellular human CD252: (52)VSHRYPR….NPGEFCVL(183).

Transfectant Cell Line:  CHO

Functional Application: CD134L-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.

References: 

1)   W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 

2)  L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 

3)  A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826.

4) V.Y.  Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627.

5) V. Malmstrom, et al, (2001) J Immunol 166: 6972.

6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115.

7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955.

8) L. Tian, et al, (2002) Transplantation 74(1): 133-138.

USD $325.00
In cart: 0
= $325.00
Catalog #:
512-020
Form:
Pur/WA
Size:
25 µg
Alternate Name:
CD252, OX40 Ligand, gp34
Applications:
EIA, FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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