anti-CD94 R-PE Conjugate

Human CD94 expression is restricted to natural killer (NK) cells, NKT cells and a subset of CD8+ T lymphocytes.  CD94 is a type II membrane glycoprotein of the c-type lectin superfamily and essentially lacks a cytoplasmic tail (4). CD94 forms a heterodimer with molecules of the NKG2 family which can recognize MHC Class Ib (HLA-E) antigen presenting molecules.

Isotype: Murine IgG1

Immunogen: Cultured human NK cells

Specificity: Antibody HP-3D9 recognizes the CD94 molecule of 70 kd (5).  It has been reported to cross react with canine CD94.

Functional Application: Antibody HP-3D9 inhibits IL-2 dependent proliferation of NK cells. It can also affect NK-cell mediated lysis of P815 cells in vitro (7). HP-3D9 blocks binding of CD94/NKG2A, B and C  heterodimers to HLA-E tetramer.

References:

1.  J. Aramburu, et al, (1990) J Immunol  144: 3238-3247.

2.  J. Aramburu, et al, (1991) J Immunol  147: 714-721.

3.  A. Moretta, et al, (1994) J Exp Med  180: 545-555.

4.  C. Chang, et al, (1995) Eur J Immunol  25: 2433-2437.

5.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1437-1439.

6.  C.S. Vetter, J.C. Becker, et al. (2000) J Invest Dermatol 114: 941-947.  PMID: 10771475

7. C Watzl, E O Long, et al (2000) J Immunol 165: 3545-3548. PMID: 11034353

8. V.M. Braud, A.J McMichael, et al. (1998) Nature 391: 795-799. doi: 10.1038/35869