anti-CD64 (F(ab’)2)

Human CD64 is a high affinity receptor for monomeric human IgG1 and IgG3 which is expressed on macrophages, monocytes, and gamma interferon induced neutrophils. CD64 plays an important role in clearance of immune complexes and in antibody dependent cytoxicity (3, 4, 5).

Isotype: Murine IgG1

Immunogen: Human rheumatoid synovial fluid cells/monocytes

Specificity: Antibody 10.1 recognizes the EC3 epitope(7) of the CD64 molecule of 72 kd from gene Fc gamma RIA (1, 3). It is cross reactive with cynomolgus monkey CD64 (8).

Functional Application: Antibody 10.1 blocks binding of Fc gamma RI to immunoglobin opsonized cells (1, 9). Incubation with anti-CD64 F(ab’)2 inhibits Fibrocyte differentiation(10) and blocks phagocytosis of antibody-opsonized platelets (11).

References:

1. G.J. Dougherty, et al, (1987) Eur J Immunol 17: 1453-1459.

2. Y. Jayaram, et al, (1989) Clin Exp Immunol 75: 414-420.

3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 874-875.

4. D.L. Durden, et al, (1995) J Immunol 154: 4039-4047.

5. L.L. Marnell, et al, (1995) J Immunol 155: 2185-2193.

6. S. von Gunten, H.U. Simon, et al. (2006) Blood 108(13): 4255-425

7. P.T.Harrison, J.M.Allen (1998) Protein Engineering 11(3):225–232.

8. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.

9. Laborde EA, M Vulcano, et al. (2007) J Immunol 179(1): 673-681.

10. Pilling D, NM Tucker, RH Gomer (2006) J Leuk Biol 79(6): 1242-1251.

11. Thijs L, Gesture Vidarsson, et al. Platelets (2022) https://doi.org/10.1080/09537104.2022.2129604 PMID: 36185007