anti-CD64 Fab-Biotin

Human CD64 is a high affinity receptor for monomeric human IgG1 and IgG3 which is expressed on macrophages, monocytes, and gamma interferon induced neutrophils.  CD64 plays an important role in clearance of immune complexes and in antibody dependent cytoxicity (3, 4, 5).

Isotype: Murine IgG1

Immunogen: Human rheumatoid synovial fluid cells/monocytes

Specificity: Antibody 10.1 recognizes the EC3 epitope(7) of the CD64 molecule of 72 kd from gene Fc gamma RIA (1, 3).  It is cross reactive with cynomolgus monkey CD64 (8).

Functional Application: Antibody 10.1 blocks binding of Fc gamma RI to immunoglobin opsonized cells (1, 9).  Incubation with anti-CD64 F(ab’)2 inhibits Fibrocyte differentiation(10).

References:

1.  G.J. Dougherty, et al, (1987) Eur J Immunol  17: 1453-1459.

2.  Y. Jayaram, et al, (1989) Clin Exp Immunol  75: 414-420.

3.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 874-875.

4.  D.L. Durden, et al, (1995) J Immunol  154: 4039-4047.

5.  L.L. Marnell, et al, (1995) J Immunol  155: 2185-2193.

6. S. von Gunten, H.U. Simon, et al. (2006) Blood 108(13): 4255-425

7. P.T.Harrison, J.M.Allen  (1998) Protein Engineering 11(3):225–232.

8. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.

9. Laborde EA, M Vulcano, et al. (2007) J Immunol 179(1): 673-681.

10. Pilling D, NM Tucker, RH Gomer (2006) J Leuk Biol 79(6): 1242-1251.

USD $925.00
In cart: 0
= $925.00
Catalog #:
216-530
Form:
Fab-Biotin
Size:
100 µg
Alternate Name:
Fcγ RI, Fc gamma RI
Clone:
10.1
Applications:
FC, WB

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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