anti-CD63 R-PE Conjugate

Human CD63 is expressed on activated platelets and also on monocytes and macrophages.  CD63 contains four hydrophobic transmembrane regions and is a member of the tetraspan family.  CD63 appears to be involved with cell adhesion and associates with VLA-3 and VLA-6 integrins (1). In a colon cancer model, its has been correlated to metastatic potential (3).

Isotype: Murine IgG1

Immunogen: Human eosinophils

Specificity: Antibody AHN16.1 recognizes the human CD63 molecule of about 50 kd (2). It also binds to equine (horse) CD63 (6).  This mAb specifically promotes adhesion of human meleanoma cells in a Ca+2 dependant manner (7).

Functional Application:  Biotinylated AHN16.1 has been used to detect and sort microvesicals from human saliva (8).

References:

1.  F. Berditchevski, et al, (1995) J Biol Chem  270: 17784-17790.

2.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1352-1364.

3.  Sordat I, Sordat B, et al. (2002) Lab Investigations 82: 1715-1724

4.  McLeish K R,  Ward R A, et al. (2013)  J Innate Immun 5: 277-289.

5.   A. Lewis,  P. T. Peachell et al. (Jan 2013) Clin Exp Allergy 43(1): 50-59.

6.  J.C. da Silveira, G.J. Bouma, et al. (2012) Biol Reproduction 86(3): 71, 1-10.  PMID 22116803

7.  J. Iida, K.M. Skubitz, et al. (2005) J Translational Med 3:42.  PMID: 16318634

8.  J Yang, DT Wong, etal. (2014) PLoS One 9(11):e110641. doi: 10.1371.  PMID: 25397880

USD $275.00
In cart: 0
= $275.00
Catalog #:
215-050
Form:
R-PE
Size:
120 tests
Alternate Name:
LIMP, LAMP-3
Clone:
AHN16.1/46-4-5
Applications:
5)), FC(4, ICC(3)

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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