anti-CD54 (D2) FITC Conjugate
Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and monocytes.
Antibodies 8.4A6 (cat #206-020) and 15.2 (cat# 205-020) each bind to a distinct epitope of CD54 and are suitable as a matched pair for EIA using 15.2 as capture and Biotinylated 8.4A6(cat# 206-030) as detector.
Isotype: Murine IgG1
Immunogen: Human endothelial cells
Specificity: Antibody 8.4A6 recognizes the D2 domain of the CD54 molecule of 90 kd (1, 2).
Functional Application: Antibody 8.4A6 inhibits CD54 binding to LFA-1.
1. A.R. Berendt, et al, (1992) Cell 68: 71-81.
2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550.
3. P.L. Reilly, et al, (1995) J Immunol 155: 529-532.