Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and monocytes.
Antibodies 8.4A6 (cat #206-020) and 15.2 (cat# 205-020) each bind to a distinct epitope of CD54 and are suitable as a matched pair for EIA using 15.2 as capture and Biotinylated 8.4A6(cat# 206-030) as detector.
Isotype: Murine IgG1
Immunogen: Human endothelial cells
Specificity: Antibody 8.4A6 recognizes the D2 domain of the CD54 molecule of 90 kd (1, 2).
Functional Application: Antibody 8.4A6 inhibits CD54 binding to LFA-1.
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