anti-CD54 (D1) FITC Conjugate

Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1).  CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes.

Antibodies 15.2 and 8.4A6 (cat #206-020) each bind to a distinct epitope of CD54 and are suitable as a matched pair for EIA using 15.2 as capture and Biotinylated 8.4A6(cat# 206-030) as detector.

Isotype: Murine IgG1 kappa

Immunogen: Human monocytes

Specificity: Antibody 15.2 recognizes the D1 domain of the CD54 molecule of 90 kd (1, 2).  It has been reported to be cross reactive with porcine CD54.

Functional Application: Antibody 15.2 inhibits CD54 binding to LFA-1.

References:

1.  A.R. Berendt, et al, (1992) Cell  68: 71-81.

2.  I. Dransfield, et al, (1992) J Cell Biol  116: 1527-1535.

3.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1548-1550.

4.  P.L. Reilly, et al, (1995) J Immunol  155: 529-532.

USD $210.00
In cart: 0
= $210.00
Catalog #:
205-040
Form:
FITC
Size:
120 tests
Alternate Name:
ICAM-1
Clone:
15.2
Applications:
EIA(capture), FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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