anti-CD49d Purified (with Antibiotics)

Human CD49d molecules are integrin alpha4 chains that are expressed as heterodimers with CD29 (beta 1 integrin) or (beta 7 integrin) and function as adhesion receptors.  CD49d/CD29 is mainly expressed on thymocytes and B cells with increased expression on activated T cells.  The ligands for CD49d/CD29 include VCAM-1 and fibronectin.

Isotype: Murine IgG1

Immunogen: Human peripheral myeloma cells

Specificity: Antibody BU49 recognizes the CD49d molecule of about 145 kd (1). It has been reported to cross react with Canine CD49d(3).

Functional Application: Antibody BU49 blocks VLA-4 binding to VCAM-1(5).  It can be used to aid in purification 0f FoxP3+ Treg cells (4).  BU49 induces IL-8 production by U-937 cells (6).

Reference:

1.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1617-1618.

2.  T. Sato, (1995) J Immunol  155: 2938-2947.

3. Ikewaki N, R Otsu, et al. (2010) Cellular Immunolgy 236(1): 55-64.

4. Kleinewietfeld M, K Falk, et al. (2012) Blood 113(4): 827-836.

5. Lakeshia J T, J L West, et al (2006) Ann Biomed Eng 34(11): 1705-1711.

6. Ikewaki N, Inoko H (2002) Microb Immunol 46(10): 685-695.

USD $175.00
In cart: 0
= $175.00
Catalog #:
200-020
Form:
Pur/WA
Size:
100 µg
Alternate Name:
VLA-4, integrinα 4
Clone:
BU49
Applications:
FC, FX

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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