anti-CD45RO FITC Conjugate

Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine     phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. CD45RO plays an essential role in lymphocyte activation (4).

Isotype: Murine IgG2a

Immunogen: Cultured human T cell line-CA-1

Specificity: Antibody UCHL1 recognizes the CD45RO isoform of about 180 kd (1, 3).  It is cross reactive with cynomolgus monkey CD45RO (7).

References:

1.S.H. Smith, et al, (1986) Immunology 58: 63-70.

2. M. Streulo, et al, (1988) J Immunol 141: 3910-3914.

3. M. Merkenschlager & P.C.L. Beverly, (1989) Intl Immunol 1: 450-459.

4. I.S. Trowbridge & M.L. Thomas, (1994) Annu Rev Immunol 12: 85-116.

5. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 386-399.

6. D. Leitenberg, et al, (1996) J Exp Med 183: 249-259.

7. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.

8.  K V Ratnam, C Y Li, et al. J Cutan Pathol (1992) 19(3):193-200. doi: 10.1111/j.1600-0560.1992.tb01658.x.

9. D.M. Menke, R.S. Kyle, et al. J Clin Path (1998) 51: 432-437

10.  Segal, Glenn H, Tubbs, Raymond R, et al. J Sug Path (1991) 15(11): 1034-1041

USD $210.00
In cart: 0
= $210.00
Catalog #:
194-040
Form:
FITC
Size:
120 tests
Alternate Name:
UCHL1, T200
Clone:
UCHL1
Applications:
10), 9, FC, FPS(8)

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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