Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. CD45RO plays an essential role in lymphocyte activation (4).
Isotype: Murine IgG2a
Immunogen: Cultured human T cell line-CA-1
Specificity: Antibody UCHL1 recognizes the CD45RO isoform of about 180 kd (1, 3). It is cross reactive with cynomolgus monkey CD45RO (7).
1.S.H. Smith, et al, (1986) Immunology 58: 63-70.
2. M. Streulo, et al, (1988) J Immunol 141: 3910-3914.
3. M. Merkenschlager & P.C.L. Beverly, (1989) Intl Immunol 1: 450-459.
4. I.S. Trowbridge & M.L. Thomas, (1994) Annu Rev Immunol 12: 85-116.
5. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 386-399.
6. D. Leitenberg, et al, (1996) J Exp Med 183: 249-259.