Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RO isoform contains none of the alternatively spliced exons and is present on most thymocytes, a subpopulation of resting (memory) T cells within both CD4 and CD8 subsets as well as on monocytes and granulocytes. CD45RO plays an essential role in lymphocyte activation (4).
Isotype: Murine IgG2a
Immunogen: Cultured human T cell line-CA-1
Specificity: Antibody UCHL1 recognizes the CD45RO isoform of about 180 kd (1, 3). It is cross reactive with cynomolgus monkey CD45RO (7).
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