anti-CD39 FITC Conjugate
Human CD39 is found on most mature B cells, activated NK cells and activated T cells, as well as Neutrophills and Monocytes(7). CD39 has homology to the N- myc family of proteins (1) and has been cloned (2). CD39 is an Ecto Apyrase(4, 6). A lower expression level of CD39 on T and NK cells have been observed in hypertensive individuals(7).
Isotype: Murine IgG1
Immunogen: Human WM-1 (Waldenström’s macroglobulinemia) cell line
Specificity: Antibody BU61 recognizes the CD39 molecule of approximately 80 kd (3). A deletion mutation study suggests that the epitope of BU61 is between amino acids 329 and 427 (11).
Applications include Immunohistochemistry (frozen section) (8), Western Blot (4,5,8,10) , Flow Cytometry, and Immunoprecipitation (3).
References:
1. J.R. Voland, et al, (1992) Proc Natl Acad Sci USA 89: 10425-10429.
2. C.R. Maliszewski, et al, (1994) J Immunol 153: 3574-3583.
3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 383-385.
4. E. Kaczmarek, et al, (1996) J Biol Chem 271:33116-33122.
5. K. Koziak, S.C. Robson, et al, (2000)J Biol Chem 275(3): 2057-2062.
6. Transfusion (May 2006) 46(6):1018-1028.
7. E D Pulte, A J Marcus, et al. (2007) Thrombosis Res 121(3): 309-317.
8. S Sheth, S. C. Robson, et al. (2009) Purinergenic Signalling 5(3): 321-326.
10. C A Haller, E L Chaikof, et al. (2006) J Vascular Surgery 43(4): 816-23. PMID: 16616242
11. J Schulte am Esch II, S C Robson, et al. (1999) Biochemistry38(8): 2248-58. PMID: 10029517.