anti-CD18 FITC Conjugate

Isotype: Murine IgG2a

Immunogen: Human peripheral blood monocytes

Specificity: Antibody IB4 recognizes the beta 2 integrin subunit (CD18) adhesion molecule of about 95 kd (1, 6).

Functional Application: Antibody IB4 blocks binding of ICAM-1 and ICAM-3 to LFA-1 (2, 5).  IB4 F(ab’)2 was used as a positive control to block adhesion of Neutrophils to recombinant CD54 (7) or A431 tumor cells (8) in vitro. IB4 blocks Trogocytic killing of Trichomonis vaginalis by human Neutrophil-like cells(9).

References:

1.  S.D. Wright, et al, (1983) Proc Natl Acad Sci USA  80: 5699-5703.

2.  R.C. Landis, et al, (1994) J Cell Biol  126: 529-537.

3.  M. Kaneko, et al, (1995) J Immunol  155: 2631-2641.

4.  A. Mazzone & G. Ricevuti, (1995) Haematologica  80: 161-175.

5.  M.E. Berman, et al, (1996) J Immunol 156: 1515-1524.

6.  K Welzenbach, G Weitz-Scmidt, et al (2002) J Biol Chem 277(12): 10590 – 10598. PMID: 11781316

7. H Conley, M K Sheats, et al. (Dec 2023)  Cell Adh Migr. 17(1): 1-17.  PMID: 37439125  doi: 10.1080/19336918.2023.2233204.

8. P Bouti, HL Matlung et al. (Dec 2023) Int J Mol Sci 24(24): 17141. https://doi.org/10.3390/ijms242417141

9. E N Trujillo, F Mercer et al. (Feb 2024) Parasite Immunology 46(2): e13025  https://doi.org/10.1111/pim.13025 PMID: 38372623