anti-CD16 Purified (with Antibiotics)

Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphatidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages.

Isotype: Murine IgG1

Immunogen: Human polymorphonuclear leukocytes

Specificity: Antibody 3G8 recognizes both allelic forms of human CD16 (1, 4). It is cross reactive with cynomolgus (7) as well as Rhesus monkey(10) CD16.

Functional Application: Antibody 3G8 blocks binding of complexed IgG to CD16 (2, 5, 9), and has been used to block ADCC in Vitro (11).   It is not suitable for Western blot.  Ancell 3G8 F(ab’2) has been used to upregulate Mac-1 expression in neutrophils (8).

References:

1.  H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA  79: 3275-3279.

2.  Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597.

3.  G. Trinchieri & N. Valiante, (1993) Natural Immunity  12: 218-234.

4.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p.  805-814.

5.  J.C. Edberg & R.P. Kimberly, (1997) J Immunol 159: 3849-3857.

6.  S. von Gunten, H.U. Simon, et al. (2006) Blood 108(13): 4255-4259.

7. Yoshino N, Honda M, et al. (2000) Exp Anim 49(2):97-110.

8.  Z Xiao, S Neelamegham, et al. (2008) Blood 112: 1091-1100.

9. Laborde EA, M Vulcano, et al. (2007) J Immunol 179(1): 673-681.

10. Choi EI, Reimann KA, et al. (2008) Immunology 124(2): 215-22 PMID: 18201184

11. H Tanaka, M Yasukawa, et al. (2016) DOI: 10.1158/1078-0432 CCR-15-2714. PMID: 27091408

USD $200.00
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Catalog #:
165-020
Form:
Pur/WA
Size:
100 µg
Alternate Name:
Fcγ RIII, Fc gamma RIII
Clone:
3G8
Applications:
FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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