Macrophages derived in culture from SLE patients are directed into secreting proinflammatory cytokines by anti-C1q auto antibodies bound to immobilized C1q complement protein. Pre blocking these monocytes with anti-CD32 (FcgRII ) F(ab’)2 reduced cytokine secretion levels significantly. “Anti-C1q Autoantibodies from Systemic Lupus Erythematosus Patients Induce a Proinflammatory Phenotype in Macrophages.”  Sophia Thanei, Marten Trendelenburg  J […]

Using TALEN(Transcription Activator-Like Effector Nucleases) technology, these researchers from Seattle Children’s Research succeeded in restoring copies of CD154( CD40L) gene with 3’ UTR under control of its endogenous promoter in human T cells from a patient with X-Linked Hyper-IgM Syndrome (X-HIGM). “Targeted gene editing restores regulated CD40L expression and function in X-HIGM T cells.” N

Neutrophil Extracellular Trap (NET) is a mechanism by which bacteria and other pathogenic organisms are rendered trapped in a site of inflammation.  An activated Neutrophil extrudes it own genomic DNA, rich with histones and other proteins, to trap invading pathogenic organisms.  However, in SLE patients, a distinct subset of Neutrophils, Low Density Granulocytes (LDGs) are

“Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1” S Jutz, P Steinberger, et al. (2016)  J Immunol Methods pii: S0022-1759(16)30007-2.  PMID: 26780292   Relevant Ancell Products   anti-human TCR Cb1 mAb   anti-human CD3e mAb CD272(BTLA) CD279(PD-1) CD137(4-1BB) anti-human CD28 (superagonist) mAb

This is distinct from recruitment of Th1 cells where CD162 is also necessary for rolling.  CD43 mediated adhesion could provide a means to selectively manipulate Th17 immune response. “CD43 Functions as an E-Selectin Ligand for Th17 Cells in Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In

These effects are Treg and IL-4 independent, and are instead dependant on the helminth specific Th2-mediated Eosinophilia in which IL-5 and IL-33 cytokines are crucial. “Helminth Products Protect against Autoimmunity via Innate Type 2 Cytokines IL-5 and IL-33, Which Promote Eosinophilia.”   Conor M Finlay, Kingston H.G. Mills, et al. (Dec 2016) J Immunol 196(2): 703-714.   

A nice review by the master. “B10 cells: a functionally defined regulatory B cell subset” T F Tedder (2015) J Immunol 194(4): 1395-1401. PMID: 25663677 Related Ancell Antibody Products anti-human CD24 anti-human CD27 anti-human CD19

Despite the pronounced proliferative effect of recombinant pentameric GITRL on T cells, MC38 adenocarcinoma tumor failed to regress due to suppressive effect of accumulating Treg  in tumor tissue and draining lymph node. “Authentic GITR Signaling Fails to Induce Tumor Regression unless Foxp3+ Regulatory T Ccells Are Depleted” Y.H. Kim, B.S. Kwon, et al. (2015) J

This Danish research group used a cocktail of Ancell Biotinylated anti tetraspan antibodies, anti-CD9, anti-CD63 and anti-CD81 to detect microvesicles captured on EV microarray.  The ability to characterize plasma derived exosomes in a high throughput manner may be very helpful in diagnosing cancer prognosis. “Potentials and capabilities of the Extracellular Vesicle (EV) Array” Malene Mǿller

Internalized ApoD is colocalized in intracellular vesicular compartments with CD147.  Downregulation of CD147 results in lower ApoD internalization.  Induction of CD147 overexpression resulted in increased ApoD internalization.  CD147 is a putative receptor for ApoD. “Apolipoprotein D Internalization Is a Basigin-dependent Mechanism” Ouafa Najyb, Louise Brissette, Eric Rassart (June 2015) J Biol Chem 290: 16077-16087. PMID: