Come and visit Ancell at the American Association of Immunologist’s meeting in Seattle May 13 – May 16th, booth #816.  Ask for an Ancell  T shirt.  They go fast!

Macrophages derived in culture from SLE patients are directed into secreting proinflammatory cytokines by anti-C1q auto antibodies bound to immobilized C1q complement protein. Pre blocking these monocytes with anti-CD32 (FcgRII ) F(ab’)2 reduced cytokine secretion levels significantly. “Anti-C1q Autoantibodies from Systemic Lupus Erythematosus Patients Induce a Proinflammatory Phenotype in Macrophages.”  Sophia Thanei, Marten Trendelenburg  J

Using TALEN(Transcription Activator-Like Effector Nucleases) technology, these researchers from Seattle Children’s Research succeeded in restoring copies of CD154( CD40L) gene with 3’ UTR under control of its endogenous promoter in human T cells from a patient with X-Linked Hyper-IgM Syndrome (X-HIGM). “Targeted gene editing restores regulated CD40L expression and function in X-HIGM T cells.” N

Neutrophil Extracellular Trap (NET) is a mechanism by which bacteria and other pathogenic organisms are rendered trapped in a site of inflammation.  An activated Neutrophil extrudes it own genomic DNA, rich with histones and other proteins, to trap invading pathogenic organisms.  However, in SLE patients, a distinct subset of Neutrophils, Low Density Granulocytes (LDGs) are

“Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1” S Jutz, P Steinberger, et al. (2016)  J Immunol Methods pii: S0022-1759(16)30007-2.  PMID: 26780292   Relevant Ancell Products   anti-human TCR Cb1 mAb   anti-human CD3e mAb CD272(BTLA) CD279(PD-1) CD137(4-1BB) anti-human CD28 (superagonist) mAb

This is distinct from recruitment of Th1 cells where CD162 is also necessary for rolling.  CD43 mediated adhesion could provide a means to selectively manipulate Th17 immune response. “CD43 Functions as an E-Selectin Ligand for Th17 Cells in Vitro and Is Required for Rolling on the Vascular Endothelium and Th17 Cell Recruitment during Inflammation In

These effects are Treg and IL-4 independent, and are instead dependant on the helminth specific Th2-mediated Eosinophilia in which IL-5 and IL-33 cytokines are crucial. “Helminth Products Protect against Autoimmunity via Innate Type 2 Cytokines IL-5 and IL-33, Which Promote Eosinophilia.”   Conor M Finlay, Kingston H.G. Mills, et al. (Dec 2016) J Immunol 196(2): 703-714.   

A nice review by the master. “B10 cells: a functionally defined regulatory B cell subset” T F Tedder (2015) J Immunol 194(4): 1395-1401. PMID: 25663677 Related Ancell Antibody Products anti-human CD24 anti-human CD27 anti-human CD19

Despite the pronounced proliferative effect of recombinant pentameric GITRL on T cells, MC38 adenocarcinoma tumor failed to regress due to suppressive effect of accumulating Treg  in tumor tissue and draining lymph node. “Authentic GITR Signaling Fails to Induce Tumor Regression unless Foxp3+ Regulatory T Ccells Are Depleted” Y.H. Kim, B.S. Kwon, et al. (2015) J

This Danish research group used a cocktail of Ancell Biotinylated anti tetraspan antibodies, anti-CD9, anti-CD63 and anti-CD81 to detect microvesicles captured on EV microarray.  The ability to characterize plasma derived exosomes in a high throughput manner may be very helpful in diagnosing cancer prognosis. “Potentials and capabilities of the Extracellular Vesicle (EV) Array” Malene Mǿller