Using transgenic mouse models and BM transplantation, it was observed that numbers of expanded thymic Treg are proportionate to number of thymic B cells present during autoimmune development. “A Role for Intrathymic B Cells in the Generation of Natural Regulatory T Cells.” Stacey N Walters, Shane Grey, et al. (2014) J Immunol 193(1): 170-176. PMID:24872190

CD273 binds to a distinct site on RGMb (Repulsive Guidance Molecule Family member B) from Bone Morphogenic proteins (BMP).  In mice, blockade of this interaction impaired development of respiratory tolerance. “RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance”  Y Xiao, G J Freeman, et al. (2014) JEM

  anti-GARP/FITC  catalog #277-040 anti-GARP/PE  catalog #277-050 anti-GARP/Biotin  catalog #277-030 anti-GARP mAb catalog #277-020   Other Ancell human Treg Products   CD4 CD39 CD357(AITR/GITR) CD45RA CD45RO CD127 CD25

This German research group has developed a method to directly culture Pre and Pro B cells from CD34+ sorted Bone Marrow using cytokines and immobilized recombinant CD54. “A Feeder-Free Differentiation System Identifes Autonomously Proliferating B Cell precursors in Human Bone Marrow”  H Kraus, M Rizzi, et al. (2014)  J Immunol 192(3): 1044-1054. PMID: 24379121  

“GARP  regulates the bioavailability and activation of TGFbeta”  R Wang, T A Springer, et al. (2012) Mol Biol Cell 23(6): 1129-1139. Look for new Ancell anti-GARP products March 2014! Ancell human Treg Products CD4 CD39 CD357(AITR/GITR) CD45RA CD45RO CD127 CD25

Visit Ancell at the 56th ASH Annual Meeting and Exposition in New Orleans Dec 7 – 9  Booth # 2671!

Agonistic humanized anti-CD134(OX40) antibodies improve T effector cell function by depleting T regulatory cells in vitro.  This approach has great potential for augmenting cancer therapy! “Antibodies Targeting Human OX40 Expand Effector T Cells and Block Inducible and Natural Regulatory T Cell Function” K.S. Voo, Y.J. Liu, et al. (2013) J Immunol 191: 3641-3650. PMID: 24014877

New Ancell Products for human Dendritic cell research! Recombinant human CD279(PD-1) (CHO cell host) Recombinant human CD273(B7-DC) (CHO cell host) anti-human CD279(PD-1) antibody (clone ANC4H6) anti-human CD273(B7-DC) antibody (clone ANC8D12)   Other related products anti-human CD274(B7-H1) antibody Recombinant human CD274(B7-H1) (CHO cell host) anti-CD11b antibody (clone ICRF44) anti-CD14 antibody (clone UCHM1)

The BTLA(CD272)-HVEM(CD270) pathway is an important regulatory mechanism for gamma/delta T cell proliferation. A subset of gamma/delta T cells can be induced to have potent anti-Lymphoma activity.  The amount of BTLA present on cell surface of these cells inversely correlates with their degree of differentiation. Blockade of this pathway can increases T cell signaling ability,

Stem Cell Factor (SCF) can activate Lung Mast cells through CD117 (c-kit) to become more responsive to IgE challenge in vitro.  Up regulation of CD63 can be used to track this activation “Heterogeneity in the responses of human lung mast cells to stem cell factor” A. Lewis, J. Wan, B. Baothman, P. N. Monk, S.