CD257(BAFF)(trn)-muCD8 Purified (Preservative-free)

The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down-regulated by mitogenic stimulation.2  BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells.3,4  Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells 1.  Cell surface BAFF can be cleaved to form a soluble trimeric molecule.2 

Molecular Structure: A soluble molecule consisting of 151 (VQGPEET…c-term) aminoacids of the extracellular domain of human BAFF fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric weight of 38.5 kd. A portion of the extracellular region of BAFF  was truncated to amino acid 135 to eliminate a potential protease cleavage site.

Transfectant Cell Line: CHO BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR (8, 9) .

Functional Application: Human BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA (8, 9,11).

References:

1) Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189(11):1747-1756.

2) Shu, H.B., H. Johnson, W.H. Hui. J Leukoc Biol 1999, 65:680-683.

3) Marsters, S.A., A. Ashkenazi, et al. 2000, Curr Biol 10:785-788.

4) Xia, X., H. Hsu, et al. 2000, J Exp Med, 192(1): 137-143.

5) Thompson J.S., C. Ambrose, et al. Science 2001, 293: 2108-2111.

6) Roschke, V, T.S. Migone, et al. J Immunol. 2002, 169: 4314-4321.

7) MacLennan, C.M., C.G. Vinuesa, 2002, Immunity 17:235-238.

8) Landau DA, P Cacoub, et al. (2009) Ann Rheum Dis 68(3): 337-44. PMID: 18434450.

9) Moreaux J, K Tarte, et al. (2004) Blood 103:3148-3157. PMID: 15070697.

10.  EY Moon, S Lee, et al. (2011) Intl Immunopharmacol 11(10): 1523-1533. https://doi.org/10.1016/j.intimp.2011.05.009

11. S Park, J Jang, EY Moon. (2020) Sci Reports 10: 11784.  https://doi.org/10.1038/s41598-020-68628-5

USD $350.00
In cart: 0
= $350.00
Catalog #:
525-820
Form:
Pur/PF
Size:
25 µg
Alternate Name:
TALL-1, Blys, THANK, B-cell Activating Factor, TNFSFL13
Applications:
EIA, FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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