anti-CD252(OX40L) Biotinylated

Human CD252 (CD134L, OX40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells.  CD252 binds to CD134(OX40) present on  activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of  inflammation and rejection (5,6,7,8).

Isotype: Murine IgG1 Kappa

Immunogen: Human HUVEC and Recombinant soluble human CD252

Specificity: Antibody ANC10G1 binds to CD252 on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.

Functional Application: Antibody ANC10G1 blocks binding of Recombinant CD134 to CD134L on HUVEC.

References:

1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763.

2)   L.M. Higgins, et al, (1999) J Immunol 162: 486-493.

3)  A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826.

4) V.Y.  Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627.

5) V. Malmstrom, et al, (2001) J Immunol 166: 6972.

6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115.

7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955.

8) L. Tian, et al, (2002) Transplantation 74(1): 133-138.

9) T Uenotsuchi, M Furue, et al. (2006) Intl Immunol 18(12): 1637-1646.

10) SM Rittig, C Hinterleitner, et al. (2022) Front. Oncol.12, (07 July 2022) https://doi.org/10.3389/fonc.2022.917834

11) A Mangelinck, V Lombardi, et al. (Jan 11, 2024) Front Immunol 14: 13201481   doi: 10.3389/fimmu.2023.1320481  PMID: 38283342

 

USD $325.00
In cart: 0
= $325.00
Catalog #:
400-030
Form:
Biotin
Size:
100 µg
Alternate Name:
CD252, OX40 Ligand, gp34, anti-OX40L, anti-OX40 Ligand
Clone:
ANC10G1
Applications:
EIA, FC(9)

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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