anti-perforin (APC)

Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis (5). Perforin expression is constitutive on NK cells, but increases in resting CD8+ cytotoxic cells upon activation.

Isotype: Murine IgG2b kappa

Immunogen: Purified (With Antibiotics) granules from human YT lymphoma cell line

Specificity: Antibody delta G9 recognizes the human and rat perforin molecule of ~70 kd (2, 4). It has been reported to bind to pre-formed perforin granules, but not granule-independent perforin(11).

References:

1. M.J. Smyth, et al, (1990) J Exp Med 171: 1269-1281.

2. A. Hameed, et al, (1992) Am J Pathol 140: 1025-1030.

3. P.L. Jose, et al, (1994) J Immunol 148: 3354-3360.

4. B.C. Schlesinger & L. Cheng, (1994) Immunol 81: 291-295.

5. C.C. Liu, et al, (1995) Immunol Today 16: 194-201.

6. A.H. Hombach, H Abken, et al. (2006) J Immunol 177: 5668-5675.

7. M.T.M. Vossen, R.A.W. van Lier, et al. (2008) J Immunol 180(6): 3739-3745.

8. Y M Kang, C M Weyand, et al (2002) J Exp Med 195(10): 1325-36. PMID: 12021312.

9. I Veneziani, E Maggi, et al. (2022) J Immunother Cancer 10(1): e003385 doi: 10.1136/jitc-2021-003385 PMID: 35091452.

10. N D Milito, Rosa Molfetta, et al. (Nov 2022) Eur J Immunol PMID: 36440686 DOI: 10.1002/eji.202250198

11. Juandy Jo, Robert Thimme, et al. (July 2012) J Hepatology 57(1): 9-16. PMID: 22425625 DOI: 10.1016/j.jhep.2012.02.030