anti-perforin FITC Conjugate

Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis (5).  Perforin expression is constitutive on NK cells, but increases in resting CD8+ cytotoxic cells upon activation.

Isotype: Murine IgG2b kappa

Immunogen: Purified (With Antibiotics) granules from human YT lymphoma cell line

Specificity: Antibody delta G9 recognizes the human and rat perforin molecule of ~70 kd (2, 4).  It has been reported to bind to pre-formed perforin granules, but not granule-independent perforin(11).

References:

1.  M.J. Smyth, et al, (1990) J Exp Med  171: 1269-1281.

2.  A. Hameed, et al, (1992) Am J Pathol  140: 1025-1030.

3.  P.L. Jose, et al,  (1994) J Immunol  148: 3354-3360.

4.  B.C. Schlesinger & L. Cheng, (1994) Immunol  81: 291-295.

5.  C.C. Liu, et al, (1995) Immunol Today  16: 194-201.

6. A.H. Hombach, H Abken, et al. (2006) J Immunol 177: 5668-5675.

7. M.T.M. Vossen, R.A.W. van Lier, et al. (2008) J Immunol 180(6): 3739-3745.

8. Y M Kang, C M Weyand, et al (2002) J Exp Med 195(10): 1325-36. PMID: 12021312.

9.  I Veneziani, E Maggi, et al. (2022) J Immunother Cancer 10(1): e003385  doi: 10.1136/jitc-2021-003385  PMID: 35091452.

10.  N D Milito, Rosa Molfetta, et al. (Nov 2022) Eur J Immunol PMID: 36440686 DOI: 10.1002/eji.202250198

11. Juandy Jo, Robert Thimme, et al. (July 2012) J Hepatology 57(1): 9-16. PMID: 22425625  DOI: 10.1016/j.jhep.2012.02.030

USD $325.00
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= $325.00
Catalog #:
358-040
Form:
FITC
Size:
120 tests
Alternate Name:
Cytolysin, deltaG9
Clone:
δG9
Applications:
FC, FC(9), FPS(2), FX, IHC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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