anti-CD154 Purified (with Antibiotics)

Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells.  Interaction of CD154 and CD40 is essential for isotype switching in B cells.  Known genetic defects that alter this interaction lead to impaired immune system function (1).  CD154  has been shown to be hyperexpressed by B and T cells in SLE patients (4).  CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells and macrophages indicating a possible role for the CD40-CD154 immunoregulatory signaling mechanism during inflammation and immunity in atherogenesis (6).

Isotype: Murine IgG1

Immunogen: Human sgp39 fusion protein

Specificity: Antibody 24-31 immunoprecipitates a CD154 (gp39) molecule of about 39 kd.  This clone has been reported to cross react with Cynomolgus and Rhesus Monkey CD154.  Binding avidity of clone 24-31 to CD154 was reported to be 8.5 nM Kd (11).

Functional Application: The antibody 24-31 will block MLR, sgp39 induced human B cell proliferation and T cell dependent B cell differentiation (9).

References:

1.  D. Gray, et al, (1994) Seminars in Immunol  6: 303-310.

2.  F. Pietravalle, et al, (1996) J Biol Chemistry  271: 5965-5967.

3.  R.J. Noelle, (1996) Immunity  4: 415-419.

4.  A. Desai-Mehta, et al, (1996) J Clin Invest  97: 2063-2073.

5.  I.S. Grewal and R.A. Flavell, (1996) Immunol Today  17: 410-414.

6.  F. Mach, et al, (1997) Proc Natl Acad Sci USA  94:1931-1936.

7. F. Pietraville, et al, (1996) Eur J. Immunol 26:725-728.

8. U.G. Wagner, C. Weyland, et al, (1998) J Immunol 161: 6390-6397.

9. M. Epeldegui, O. Martinez-Maza, et al. (2010) PLOS one 5(7): e11448. PMI11.D: 20625427.

10. B Barnhart, L R Covey, et al. (2000) Immunology 99(1): 54-61. PMID: 10651941

11.  P. Brams, R Newman, et al (2001) Int Immunopharmacol 1(2):277-94. doi: 10.1016/s1567-5769(00)00020-5

USD $210.00
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= $210.00
Catalog #:
353-020
Form:
Pur/WA
Size:
100 µg
Alternate Name:
CD40L, CD40 Ligand, gp39
Clone:
24-31
Applications:
6), 7), EIA, FC, IHC(8), WB(2

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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