anti-CD44 (clone BU75) Purified (Preservative-free)

Human CD44 molecules appear to serve as lymphocyte adhesion molecules with numerous functions in extracellular matrix binding, cell migration, hemopoisis, and lymphocyte homing (1).

Isotype: Murine IgG2a

Immunogen: Human peripheral myeloma cells

Specificity: Antibody BU75 recognizes epitope 2 of the CD44 molecule which interacts with hyaluronate (HA)mediating binding of lymphocytes to high endothelial venules (2, 3). This epitope appears to be conformation dependant (4).

Functional Application: Antibody BU75 blocks binding of HA to CD44 (2,7, 9), Induces FasL expression in T cells (6), and inhibits DNA sythesis and IL2R expression in activated T cells (5).

References:

1.  B.F. Hynes, et al, (1993) Adv Immunol  54: 271-335.

2.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1713-1742.

3.  H.X. Liao, et al, (1995) J Immunol  155: 3938-3945.

4.  J. Bajorath, A. Aruffo, et al. (1998) J Biol Chem  273: 338-343.

5.  Sugiyama K, M Sakurai, et al (1999) Immunol Invest 28(2,3): 185-200.

6.  Nakano, K, Y Tanaka, et al (2007) Apoptosis 12(1): 45-54.

7. Y Fujii, Y Tanaka, et al. (2003) FEBS Letters 539 (1-3): 45-50. PMID: 12650924.

8. H Kawashima, M Miyasaka, et al (2000) J Biol Chem 275: 35448-56. PMID: 10950950

9. T Tamura, H Sato, et al. (2016) Arthrits Res Ther 18(1): 121. PMID: 27039182.

USD $175.00
In cart: 0
= $175.00
Catalog #:
352-820
Form:
Pur/PF
Size:
100 µg
Clone:
BU75
Applications:
FC, WB(3)

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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