Isotype: Mouse IgG1k
Immunogen: Recombinant human Hif1a
INFORMATION: Hypoxia Inducible Factor 1 alpha (Hif-1a) is a transcription factor which accumulates in the nucleus of cells in most tissues which are subjected to hypoxic conditions. There it stabilizes by associating with Hif-1 beta (ARNT).
Clone ANC10G3 binds to recombinant human Hif-1a in EIA, as well as native HIf1a present in nuclei of fixed permeabolized Nalm-6 cells cultured overnight with CoCl2 to simulate hypoxia. It interferes with binding of anti-Hif1a clone 546-16, suggesting that their epitopes overlap.
Hif1 References: 1) GL Wang, GL Song, et al. (1993) PNAS 90: 4304