anti-CD94 Biotinylated

Human CD94 expression is restricted to natural killer (NK) cells, NKT cells and a subset of CD8+ T lymphocytes.  CD94 is a type II membrane glycoprotein of the c-type lectin superfamily and essentially lacks a cytoplasmic tail (4). CD94 forms a heterodimer with molecules of the NKG2 family which can recognize MHC Class Ib (HLA-E) antigen presenting molecules.

Isotype: Murine IgG1

Immunogen: Cultured human NK cells

Specificity: Antibody HP-3D9 recognizes the CD94 molecule of 70 kd (5).  It has been reported to cross react with canine CD94.

Functional Application: Antibody HP-3D9 inhibits IL-2 dependent proliferation of NK cells. It can also affect NK-cell mediated lysis of P815 cells in vitro (7). HP-3D9 blocks binding of CD94/NKG2A, B and C  heterodimers to HLA-E tetramer.

References:

1.  J. Aramburu, et al, (1990) J Immunol  144: 3238-3247.

2.  J. Aramburu, et al, (1991) J Immunol  147: 714-721.

3.  A. Moretta, et al, (1994) J Exp Med  180: 545-555.

4.  C. Chang, et al, (1995) Eur J Immunol  25: 2433-2437.

5.  Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1437-1439.

6.  C.S. Vetter, J.C. Becker, et al. (2000) J Invest Dermatol 114: 941-947.  PMID: 10771475

7. C Watzl, E O Long, et al (2000) J Immunol 165: 3545-3548. PMID: 11034353

8. V.M. Braud, A.J McMichael, et al. (1998) Nature 391: 795-799. doi: 10.1038/35869

USD $325.00
In cart: 0
= $325.00
Catalog #:
315-030
Form:
Biotin
Size:
100 µg
Alternate Name:
Kp43, KLRD1
Clone:
HP-3D9
Applications:
FC, IHC(6)

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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