anti-CD81 Biotinylated

Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion, and cellular activation (1).  Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation (2, 3).  Work with CD81-null mice indicates CD81 is important for development of humoral immune response (4). The level of cell surface CD81 decreases rapidly upon activation of T or B cell, and appears to be shed off in exosomes (6).  CD81 colocalizes with intracellular HIV-1 containing vesicals (5) and interacts with Gag viral assembly protiens(7).

Isotype: Murine IgG1

Immunogen: Human B cell line from Burkitt lymphoma

Specificity: Antibody 1.3.3.22 reacts with the 24 kd tetraspan CD81 molecule (1).  The binding site of this mAb interferes with the binding of Hepatiatis C E2 glycoprotein to CD81 and may involve Leu162 (8).

References:

1.  Leukocyte Typing V (S.F. Schlossman, et al, eds.)  Oxford University Press, Oxford  (1995) p. 684-691.

2.  B.A. Mannion, et al, (1996) J Immunol  157: 2039-2047.

3.  S.C. Todd, et al, (1996) J Exp Med  184: 2055-2060.

4.  H.T. Maecker & S. Levy, (1997) J Exp Med  185: 1505-1510.

5.  H. Koppensteiner, M. Schuler, et al. (2012) J Virol 86(5): 2826-2836.

6.  B. Fritzsching, M. Ott, et al. (2002) J Immunol 169(10): 5531-5537.

7.  B Grigorov, D. Muriaux, et al. (2009) Retrovirol 6: 28.

8. H.E. Drummer, K.A. Wilson, P. Poumbourios (2002) J Virol 76(21): 11143-11147. PMID: 12368358

USD $210.00
In cart: 0
= $210.00
Catalog #:
302-030
Form:
Biotin
Size:
100 µg
Alternate Name:
TAPA-1
Clone:
1.3.3.22
Applications:
6), FC, ICC(5), WB(6)

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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