anti-CD279 (PD-1) Purified (with Antibiotics)

Isotype:  Murine IgG1k

Immunogen: Recombinant human CD279

INFORMATION: Human CD279 (PD-1, Programmed death 1) is a 55 kd Ig superfamily member  with sililarity to CD28 and CD152(CTLA-4).    It is expressed on activated T and B and myeloid cells, and engagement by its ligands PD-L1 (CD274, B7-H1) or PD-L2 (B7-DC) can inhibit proliferation and cytokine expression.  In mice blockade of PD-1 ligand interaction has been used to augment T cell anti cancer responses (3,4,5).

Anti-CD279 antibody clone ANC4H6 binds to CD279 present on PHA activated PBMC in FACS, and binds to recombinant CD279 in EIA.  Pre incubation of recombinant CD279-coated plates with ANC4H6 blocks binding of biotinylated recombinant CD273  in EIA.

References:

1) Ishida et al. (1992) EMBO J 11(11):3887-95.

2) Carreno BM, M Collins, et al. (2001) Annu Rev Immunol 20:29.

3) MA Curran, Allison JP, et al. (2010) PNAS 107(9): 4275-80.

4) Mangsbo SM, TH Totterman, et al. (2010) J Immunotherapy 33(3):225.

5) Hirano F, L Chen, et al. (2005) Canc Res 65: 1089.

USD $175.00
In cart: 0
= $175.00
Catalog #:
279-020
Form:
Pur/WA
Size:
100 µg
Alternate Name:
PD1, Programmed Death Receptor 1
Clone:
ANC4H6
Applications:
EIA, FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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