anti-CD278 (ICOS) FITC Conjugate

The inducible costimulator CD278 (ICOS) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or CD278 results in discrete cytokine secretion profiles by the activated T cells.1 Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement.2 Human CD275 (ICOSL, GL50, B7-H2) is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for CD278 (ICOS).3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. 4

Isotype: Murine IgG1 Kappa

Immunogen: Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg

Specificity: Antibody ANC6C6 binds to recombinant and cell surface CD278(ICOS)

Functional Application: Antibody ANC6C6 blocks binding of Recombinant CD275-muIg to CD278.  It blocks CD28-superagonist/CD275(LICOS) mediated T cell proliferation in vitro(7).

References:

1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717.

2) Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948.

3) Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657.

4) Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308.

5) Tomoki Ito, Yong-Jun Liu, et al. (2008) Immunity 28: 870-880.

6) Hyung W Lim, Chang H Kim. (2007) J Immunol 179: 7448-7456.

7) S Weissmuller, Z Waibler, et al. (2012) Blood 119(26):6268-6277.

USD $300.00
In cart: 0
= $300.00
Catalog #:
265-040
Form:
FITC
Size:
120 tests
Alternate Name:
ICOS, AILIM, anti-ICOS
Clone:
ANC6C6-A3
Applications:
6), EIA, FC, IHC(5)

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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