anti-CD275 Purified (with Antibiotics)

The inducible costimulator CD278 (ICOS) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Unlike CD28, CD278 is only expressed on activated T cells. Secondary signaling through CD28 or CD278  results in discrete cytokine secretion profiles by the activated T cells(1).  Engagement of CD152 (CTLA-4) anergizes cells costimulated with either CD28 and CD278(2). Signaling through CD278 is particularly important in progression of  TH2 immune response (5). The receptor for human CD278(ICOS) is CD275(GL50,ICOS L), a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2)(3). Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain(4).  Blockade of the CD278-CD275 interaction in mice improves allograft survival(6) and reduces EAE(7). 

Isotype: Murine IgG1 Kappa

Immunogen: Human Raji cells, recombinant human CD275-muIg

Specificity: Antibody ANC4E3 binds to recombinant CD275 in EIA.  It does not block the interaction between recombinant CD278 and CD275.

References:

1)  Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717.

2)   Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948.

3)   Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657.

4)   Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308.

5)   K.C. Beier, et al, (2000) Eur J Immunol 30: 3707-3717.

6)   E. Ozkaynak, et al, (2001) Nat Immunol 2: 591-596.

7)   J.B. Rottman, et al, (2001) Nat Immunol 2: 605-611.

USD $210.00
In cart: 0
= $210.00
Catalog #:
263-020
Form:
Pur/WA
Size:
100 µg
Alternate Name:
ICOSL, GL50, B7-H2
Clone:
ANC4E3
Applications:
EIA

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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