anti-CD66b (ANC1D5) FITC Conjugate

Human CD66b (CEACAM8, CGMb, NCA-95) is a 95kD member of the Carcinoembryonic Antigen (CEA) group. It is expressed on granulocytes and functions as an adhesion molecule.  Similar to the CD66 a, c, and d isoforms, engagement of CD66b on neutrophils causes increased adhesion to endothelial cells (2).  Crosslinking CD66b on neutrophils with antibodies triggers IL-8 release (3).

Isotype:
Murine IgG1k

Immunogen: Recombinant human soluble CD66b

Specificity:
Antibody from clone ANC1D5 binds to CD66b on the cell surface of human PMN in Flow cytometry and detects recombinant CD66b in sandwich EIA utilizing either clone ANC4A4(Cat #220-020) or ANC3C5(Cat# 219-020) as a capture reagent.  Clone ANC1D5 may cross react with other CD66 isoforms, as it also binds to cell surface of an epithelial tumor line.

References:

1) W.N. Khan, S. Hammarstrom, et al. (1992) Genomics 14(2):384-90.

2) K.M. Skubitz, A.P. Skubitz, et al. (1996) J Leuk Biol 60(1): 106-117.

3) A.K. Schroder, L Rink, et al. (2006) Human Immunol 67(9): 676-682.

USD $325.00
In cart: 0
= $325.00
Catalog #:
217-040
Form:
FITC
Size:
120 tests
Alternate Name:
CEACAM8, CGMb, NCA-95
Clone:
ANC1D5
Applications:
EIA(detect), FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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