anti-CD32 R-PE Conjugate

Human CD32 exists in at least six isoforms originating from three different genes (Fc gamma RII A, B, and C) .  The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses.

Isotype: Murine IgG1k

Immunogen: Human K562 tumor cells and L cells transfected with human Fc gamma RII

Specificity: Antibody 7.3 reacts with a domain 2 epitope of all CD32 isoforms (1). It exhibits preferential binding to  Fc gRIIb (inhibitory) isoform transfectants(2, 5).

Functional Application: Intact and F(ab’)2  7.3 antibody blocks immune complex binding (4).  Incubation with anti-CD32 F(ab’)2 inhibits Fibrocyte differentiation(6), and inhibits pro inflammatory cytokine secretion by SLE patient derived monocytes (7).

References:

1.   Leukocyte Typing V (S.F. Schlossman, et al, eds.)  Oxford University Press, Oxford  (1995)  p. 823-836.

2.  Liu Y, L Pricop, et al.  (2005) J Leukocyte Biol 77: 767-776 PMID:15703199

3.  S. von Gunten, H.U. Simon, et al. (2006) Blood 108(13): 4255-425

4. Laborde EA, M Vulcano, et al. (2007) J Immunol 179(1): 673-681.

5. Ierino FL, P M Hogarth, et al. (1993) J Immunol 150(5): 1794-1803.

6. Pilling D, NM Tucker, RH Gomer (2006) J Leuk Biol 79(6): 1242-1251.

7. Thanei S,  M Trendelenburg  (2016) J Immunology 196: 2063–2074. PMID: 26829984

USD $275.00
In cart: 0
= $275.00
Catalog #:
181-050
Form:
R-PE
Size:
120 tests
Alternate Name:
Fcγ RII, Fc gamma RII
Clone:
7.3
Applications:
FC

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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