anti-CD4 (D1) FITC Conjugate

Human CD4 is a cell surface glycoprotein of 55 kd expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells.  CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells (3).  The extracellular portion of CD4 is an array of four Ig-like domains.  The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120.

Antibodies QS4120 and M-T441 (cat #148-020) each bind to a distinct epitope of CD4 and are suitable as a matched pair for EIA using QS4120 as capture and Biotinylated M-T441(cat# 148-030) as detector.

Isotype: Murine IgG1

Immunogen: CD4+ transfectant/human CEM

Specificity: Antibody QS4120 recognizes an extracellular epitope in the CDR2-like loop within domain 1 region of the CD4 molecule (6).

Functional Application: Antibody QS4120 will block binding of HIV-1 gp120 protein to CD4 (4) and also blocks HLA Class II Rosette formation.

References:

1.  D. Healey, et al, (1990) J Exp Med 172: 1233-1242.

2.  Leukocyte Typing V (S.F. Schlossman, et al, eds.)  Oxford University Press, Oxford, (1995)  p. 476-478.

3.  R. König, et al, (1995) J Exp Med  182: 779-787.

4.  S. Ugolini, et al, (1997) J Immunol  159: 3000-3008.

5.  S. Dornan et al, (2002) J Biol Chem 277(3): 1912.

6.  S.E.M. Howie, D.J. Harrison, et al. (1999) FASEB 13(8): 503-511. PMID: 10064617.

USD $210.00
In cart: 0
= $210.00
Catalog #:
147-040
Form:
FITC
Size:
120 tests
Alternate Name:
Leu3A, T4
Clone:
QS4120
Applications:
Blocking, EIA(capture), FC, FX

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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