anti-MHC Class I R-PE Conjugate

Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells (2).  MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane alpha chain and an invariant beta chain.  MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells.  Evidence indicates exogenously derived peptides are also presented by MHC Class I molecules (4).

Isotype: Murine IgG2a

Immunogen: Human HSB-2 T cell line

Specificity: Antibody 3F10 recognizes a monomorphic Class I epitope on the alpha chain (1). It has been reported to cross react with canine Class I (9,10).

Functional Application: Antibody 3F10 (whole molecule) is cytotoxic in the presence of complement (1).

References:

1.  GS Eisenbarth, et al, (1980) J Immunol  124: 1237-1244.

2.  PJ Bjorkman & P Parham, (1990) Annu Rev Biochem  59: 253-288.

3.  LD Barber & P Parham, (1994) J Exp Med  180: 1191-1194.

4.  KL Rock, (1996) Immunol Today  17: 131-137.

5.  J Immunol (1985) 135(3): 1752-1759.

6. A Kawana-Tachikawa, A Iwamoto, et al (2002) J Virol 76(23):11982-11988.

7. JE Shaw, RW Lang, et al (2001) J Virol Meth 94: 137-146.

8. RM McCallum, BF Haynes, et al. (1993) Opthalmol Vis Sci 34: 1793-1803

9. T.J. Yang, JP Chandler & S. Dunne-Anway. 1987 Br J Cancer 55: 131-134.

10. P Ross, RR Hess, et al. (Dec 2012) Vet Immunol Immunopathol 150(3,4): 206-212  doi: 10.1016/j.vetimm.2012.08.012. Epub 2012 Sep 21.

USD $275.00
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= $275.00
Catalog #:
121-050
Form:
R-PE
Size:
120 tests
Alternate Name:
HLA-A, HLA-B, HLA-C, Major Histocompatibility complex
Clone:
3F10
Applications:
EIA(6), FC(7), IHC(5

Application Key

FC
Flow Cytometry
WB
Western blot
EIA
Enzyme Immunoassay
IHC
Immunohistochemistry
ICC
Immunocytochemistry
FPS
Fixed paraffin section
PS
Paraffin section
IP
Immunoprecipitation
FX
Fixed cells or tissues

Applications indicated have been either tested in-house or reported. Products may be functional for applications not indicated.

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