anti-MHC Class I (R-PE)

Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells (2). MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane alpha chain and an invariant beta chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Evidence indicates exogenously derived peptides are also presented by MHC Class I molecules (4).

Isotype: Murine IgG2a

Immunogen: Human HSB-2 T cell line

Specificity: Antibody 3F10 recognizes a monomorphic Class I epitope on the alpha chain (1). It has been reported to cross react with canine Class I (9,10).

Functional Application: Antibody 3F10 (whole molecule) is cytotoxic in the presence of complement (1).

References:

1. GS Eisenbarth, et al, (1980) J Immunol 124: 1237-1244.

2. PJ Bjorkman & P Parham, (1990) Annu Rev Biochem 59: 253-288.

3. LD Barber & P Parham, (1994) J Exp Med 180: 1191-1194.

4. KL Rock, (1996) Immunol Today 17: 131-137.

5. J Immunol (1985) 135(3): 1752-1759.

6. A Kawana-Tachikawa, A Iwamoto, et al (2002) J Virol 76(23):11982-11988.

7. JE Shaw, RW Lang, et al (2001) J Virol Meth 94: 137-146.

8. RM McCallum, BF Haynes, et al. (1993) Opthalmol Vis Sci 34: 1793-1803

9. T.J. Yang, JP Chandler & S. Dunne-Anway. 1987 Br J Cancer 55: 131-134.

10. P Ross, RR Hess, et al. (Dec 2012) Vet Immunol Immunopathol 150(3,4): 206-212 doi: 10.1016/j.vetimm.2012.08.012. Epub 2012 Sep 21.