This German research group has developed a method to directly culture Pre and Pro B cells from CD34+ sorted Bone Marrow using cytokines and immobilized recombinant CD54.
“A Feeder-Free Differentiation System Identifes Autonomously Proliferating B Cell precursors in Human Bone Marrow” H Kraus, M Rizzi, et al. (2014) J Immunol 192(3): 1044-1054. PMID: 24379121
Relevant Ancell Products
Recombinant human CD54(ICAM-1)-muIg
Flow Cytometry Human Phenotyping Reagents