room34

Ancell anti-CD28 superagonist mAb used to expand Treg cells

“Single CD28 stimulation induces stable and polyclonal expansion of human regulatory T cells” Xuehui He, Ruben L Smeets,  Esther van Rijssen, Annemieke MH Boots, Irma Joosten, Hans JPM Koenen.  (2016) Dissertation Submitted for Publication,  Radbound Univ Med Center and Groningen Univ Med Center, The Netherlands. Ancell anti-CD28 Superagonist  Clone ANC28.1/5D10

Ancell anti-CD28 superagonist mAb used to expand Treg cells Read More »

anti-CD32 F(ab’)2 blocked receptors on SLE patient-derived stimulated monocytes and reduced proinflammatory cytokine secretion in vitro

Macrophages derived in culture from SLE patients are directed into secreting proinflammatory cytokines by anti-C1q auto antibodies bound to immobilized C1q complement protein. Pre blocking these monocytes with anti-CD32 (FcgRII ) F(ab’)2 reduced cytokine secretion levels significantly. “Anti-C1q Autoantibodies from Systemic Lupus Erythematosus Patients Induce a Proinflammatory Phenotype in Macrophages.”  Sophia Thanei, Marten Trendelenburg  J

anti-CD32 F(ab’)2 blocked receptors on SLE patient-derived stimulated monocytes and reduced proinflammatory cytokine secretion in vitro Read More »

TALEN gene editing used to restore normal CD154 expression and function in X-HIGM human T cells

Using TALEN(Transcription Activator-Like Effector Nucleases) technology, these researchers from Seattle Children’s Research succeeded in restoring copies of CD154( CD40L) gene with 3’ UTR under control of its endogenous promoter in human T cells from a patient with X-Linked Hyper-IgM Syndrome (X-HIGM). “Targeted gene editing restores regulated CD40L expression and function in X-HIGM T cells.” N

TALEN gene editing used to restore normal CD154 expression and function in X-HIGM human T cells Read More »

Oxidized Mitochondrial products in NETosis are a major component by which LDG drive inflammation in SLE

Neutrophil Extracellular Trap (NET) is a mechanism by which bacteria and other pathogenic organisms are rendered trapped in a site of inflammation.  An activated Neutrophil extrudes it own genomic DNA, rich with histones and other proteins, to trap invading pathogenic organisms.  However, in SLE patients, a distinct subset of Neutrophils, Low Density Granulocytes (LDGs) are

Oxidized Mitochondrial products in NETosis are a major component by which LDG drive inflammation in SLE Read More »

Engineered T cell reporter line may further elucidate T cell signal pathway intricacies

“Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1” S Jutz, P Steinberger, et al. (2016)  J Immunol Methods pii: S0022-1759(16)30007-2.  PMID: 26780292   Relevant Ancell Products   anti-human TCR Cb1 mAb   anti-human CD3e mAb CD272(BTLA) CD279(PD-1) CD137(4-1BB) anti-human CD28 (superagonist) mAb

Engineered T cell reporter line may further elucidate T cell signal pathway intricacies Read More »